Local-first bioinformatics software for DTC DNA raw data conversion, standard VCF/gVCF variant processing, genomic variant annotation, and research-grade desktop and CLI pipelines.
Note
Machine-Readable Context & Index
This organization profile serves as the central landing page and public repository directory for biotec-line. All genetic processing, variant annotation, reference genome matching, and file parsing operate strictly local on the user's hardware. For AI agents, search engines, and automated crawlers, see llms.txt.
biotec-line builds practical, privacy-centric Python desktop software for working with sensitive genetic files on local machines. The current projects focus on converting direct-to-consumer (DTC) DNA exports (23andMe, MyHeritage, FamilyTreeDNA, etc.) into standard VCF 4.2, validating genome builds (GRCh37/GRCh38), annotating VCF/gVCF data with gnomAD and ClinVar fields, filtering variants, and exporting research-oriented reports without routing genomic data into third-party cloud infrastructure.
The organization profile indexes all 3 public biotec-line repositories, verified against GitHub metadata on 2026-07-30.
| Repository | Latest public push | Public focus |
|---|---|---|
| VFDistiller | 2026-07-28 | Local-first VCF/gVCF annotation, filtering, validation, dependency audit, gnomAD/ClinVar-oriented fields, and research export desktop workflows |
| genotype-to-vcf | 2026-07-26 | DTC DNA raw-data to VCF 4.2 conversion with PySide6 GUI, headless CLI, GRCh37/GRCh38 detection, dbSNP, and FASTA reference lookup |
| .github | 2026-07-30 | Public organization start page, shared community-health defaults, and machine-readable llms.txt discovery context |
The biotec-line ecosystem provides an offline-first pipeline for processing and annotating direct-to-consumer genomic data:
graph TD
Input[DTC DNA Raw Data<br/><i>23andMe, MyHeritage, FTDNA, etc.</i>] --> G2V(genotype-to-vcf)
G2V -->|dbSNP / FASTA Ref Lookup| BuildDetect{Auto-Detect Build}
BuildDetect -->|GRCh37 / GRCh38| OutVCF[Standard VCF 4.2 File]
OutVCF --> VFD(VFDistiller)
VFD -->|Local Cache / DBs| Annotate{Annotation & Filtering}
Annotate -->|gnomAD / ClinVar / VEP| Reports[Research Reports<br/><i>Excel, CSV, PDF, VCF</i>]
classDef default fill:#1e1e2e,stroke:#45475a,stroke-width:2px,color:#cdd6f4;
classDef tool fill:#1e3a8a,stroke:#3b82f6,stroke-width:2px,color:#eff6ff;
classDef data fill:#313244,stroke:#585b70,stroke-width:1px,color:#cdd6f4;
class G2V,VFD tool;
class Input,OutVCF,Reports data;
| Need | Start with | Why |
|---|---|---|
| Convert 23andMe, MyHeritage, FTDNA, or similar raw DNA exports to VCF 4.2 | genotype-to-vcf | PySide6 desktop converter and headless CLI with GRCh37/GRCh38 detection, dbSNP/FASTA reference lookup, SHA256 release verification, local cache, and privacy-focused VCF output. Runs on Windows, macOS, and Linux. |
| Annotate, filter, inspect, and export VCF/gVCF or 23andMe-derived variant files | VFDistiller | Local-first variant analysis GUI with gnomAD, MyVariant.info, VEP, ALFA, TOPMed, ClinVar-oriented fields, FASTA validation, CSV/Excel/PDF/VCF export, dependency audit, and cross-platform CI smoke tests. |
This directory lists all 3 public repositories that are part of the biotec-line organization profile as verified on 2026-07-30. Private, internal, or unreleased research work is intentionally not listed here.
| Repository | Role | Public status |
|---|---|---|
| .github | Organization profile, shared issue templates, contribution policy, security policy, and machine-readable llms.txt context |
Active |
| genotype-to-vcf | DTC DNA raw-data to VCF 4.2 converter for 23andMe, MyHeritage, FTDNA, GRCh37/GRCh38, dbSNP, FASTA, SHA256 release checksums, and CLI pipeline workflows | Active |
| VFDistiller | Local-first VCF/gVCF annotation, filtering, validation, dependency audit, gnomAD/ClinVar-oriented fields, FASTA-oriented review, and export desktop tool for research-use-only variant workflows | Active |
| Project | Description |
|---|---|
| genotype-to-vcf | Converts four-column DTC DNA raw data into VCF 4.2 with automatic build detection, sex-aware ploidy handling, dbSNP integration, optional offline FASTA lookup, headless CLI mode for macOS/Linux pipelines, SHA256SUMS release verification, and local-only file handling. |
| Project | Description |
|---|---|
| VFDistiller | Local-first desktop app for VCF, gVCF, 23andMe raw text, and FASTA workflows, including annotation, filtering, allele-frequency lookup, reference validation, research exports, and cross-platform source smoke tests (Windows, macOS, Linux). |
Important
Research Use Only
These tools are built for bioinformatics research, teaching, software development, and reproducible local workflows. They are not medical devices, not in-vitro diagnostic products, not clinically validated, and not intended for diagnosis, prognosis, therapy decisions, or clinical interpretation of genetic results.
Users remain responsible for lawful handling of genetic data, informed consent, data minimization, local storage security, and interpretation by qualified professionals where health-related questions are involved.
- Local first: Raw genetic files, generated VCFs, caches, reference genomes, and settings stay on the user's machine by default.
- Privacy-conscious: Tools avoid transmitting genotype data or raw files; optional external calls are limited and documented, such as rsID lookup or reference genome downloads.
- Research-use clarity: README files and application surfaces keep the non-clinical, non-diagnostic boundary visible.
- Cross-platform: Desktop GUI on Windows; headless CLI for macOS and Linux pipeline integration.
- Inspectable data flow: Conversion, annotation, filtering, caches, ignored local artifacts, and export formats are documented for maintainers and LLM-assisted review.
For crawlers and LLM tools, see llms.txt. It lists canonical repositories, project roles, research-use boundaries, and preferred search phrases for the biotec-line organization.
Useful GitHub and web search phrases include biotec-line VCF tools, DTC DNA to VCF converter, 23andMe MyHeritage FTDNA VCF conversion, local-first VCF annotation desktop app, genotype-to-vcf CLI headless pipeline, gVCF filtering GUI, gnomAD ClinVar FASTA variant review, research-use-only genetic variant annotation software, offline bioinformatics desktop tools, and privacy-first genomic data conversion.
biotec-line is the bioinformatics branch of the broader research and AI tool ecosystem:
| Organization | Purpose | Link |
|---|---|---|
| open-bricks | Umbrella organization for all tools and software products | open-bricks |
| research-line | Open-Science and research repositories | research-line |
| ellmos-ai | AI Infrastructure and Agent frameworks | ellmos-ai |
| doc-bricks | Document management and processing tools | doc-bricks |
| dev-bricks | Developer tools, bridges, and utilities | dev-bricks |
| file-bricks | File management and desktop applications | file-bricks |
| lukisch | Personal developer profile | lukisch |